CIFOR-ICRAF s’attaque aux défis et aux opportunités locales tout en apportant des solutions aux problèmes mondiaux concernant les forêts, les paysages, les populations et la planète.

Nous fournissons des preuves et des solutions concrètes pour transformer l’utilisation des terres et la production alimentaire : conserver et restaurer les écosystèmes, répondre aux crises mondiales du climat, de la malnutrition, de la biodiversité et de la désertification. En bref, nous améliorons la vie des populations.

CIFOR-ICRAF publie chaque année plus de 750 publications sur l’agroforesterie, les forêts et le changement climatique, la restauration des paysages, les droits, la politique forestière et bien d’autres sujets encore, et ce dans plusieurs langues. .

CIFOR-ICRAF s’attaque aux défis et aux opportunités locales tout en apportant des solutions aux problèmes mondiaux concernant les forêts, les paysages, les populations et la planète.

Nous fournissons des preuves et des solutions concrètes pour transformer l’utilisation des terres et la production alimentaire : conserver et restaurer les écosystèmes, répondre aux crises mondiales du climat, de la malnutrition, de la biodiversité et de la désertification. En bref, nous améliorons la vie des populations.

CIFOR–ICRAF publishes over 750 publications every year on agroforestry, forests and climate change, landscape restoration, rights, forest policy and much more – in multiple languages.

CIFOR–ICRAF addresses local challenges and opportunities while providing solutions to global problems for forests, landscapes, people and the planet.

We deliver actionable evidence and solutions to transform how land is used and how food is produced: conserving and restoring ecosystems, responding to the global climate, malnutrition, biodiversity and desertification crises. In short, improving people’s lives.

Induction and regeneration of somatic embryos from Vitex doniana (Lamiaceae) leaf explants

Exporter la citation

The present study was conducted with the aim of evaluating some of the factors that influence induction and regeneration of somatic embryos in Vitex doniana since there are no available reports on tissue culture of this tree species. Leaves from plants growing under temporary shed were cultured on Murashige and Skoog supplemented with silver nitrate and four amino acids (proline, tryptophan, lysine and leucine) at varying concentrations, 0.11 mg/l thidiazuron, 2% sucrose and 100 mg/l myo-inositol in separate experiments. The explants cultured on media supplemented with tryptophan at 30.6 mg/l produced the optimal (6.5) number of embryos per explant. This number was fivefold more than the number obtained in the control. On the other hand, it was observed that the explants on media supplemented with silver nitrate at 8.45 mg/l gave the same mean (6.5) number of embryos per explant. These first ever results on the induction of somatic embryo in V. doniana could be used for mass propagation and to select useful traits of this tree species at the cellular level. However, further work needs to be done on the conversion of the regenerated embryos.

DOI:
https://doi.org/10.5897/IJBMBR2014.0216
Dimensions Nombre de citations:

Publications connexes