The bovine mammary gland is composed of various cell types including bovine mammary epithelial cells (BMEC). The use of BMEC to uncover the microRNA (miRNA) profile would allow us to obtain a more specific profile of miRNA sequences that could be associated with lactation and avoid interference from other cell types. The objective of this study was to characterize the miRNA sequences expressed in isolated BMEC. The miRNA were identified by Solexa sequencing technology (Illumina Inc., San Diego, CA). Furthermore, novel miRNA were uncovered by stem-loop reverse transcription-PCR and sequencing of PCR products. To detect tissue specificity, expression of novel miRNA sequences was measured by stem-loop RT-PCR and sequencing of PCR products in mammary gland, liver, adipose, ileum, spleen and kidney tissue from 3 lactating Holstein cows (50 ± 10 d postpartum). After bioinformatics analysis, 12,323,451 reads were obtained by Solexa sequencing, of which 11,979,706 were clean reads, matching the bovine genome. Among clean reads, 9,428,122 belonged to miRNA sequences. Further analysis revealed that the miRNA bta-mir-184 had the most abundant expression, and 388 loci possessed the typical stem-loop structures matching known miRNA hairpins. In total, 38 loci with novel hairpins were identified as novel miRNA and were numbered from bta-U1 to bta-U38. One novel miRNA (bta-U21) was specific to mammary gland. Seven novel miRNA, including bta-U21, had tissue-restricted distribution. Uncovering the specific roles of these novel miRNA during lactation appears warranted
CIFOR-ICRAF aborda retos y oportunidades locales y, al mismo tiempo, ofrece soluciones a los problemas globales relacionados con los bosques, los paisajes, las personas y el planeta.
Aportamos evidencia empírica y soluciones prácticas para transformar el uso de la tierra y la producción de alimentos: conservando y restaurando ecosistemas, respondiendo a las crisis globales del clima, la malnutrición, la pérdida de biodiversidad y la desertificación. En resumen, mejorando la vida de las personas.
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Explore eventos futuros y pasados organizados por CIFOR-ICRAF o con la participación de nuestros investigadores.
CIFOR-ICRAF produce cada año más de 750 publicaciones sobre agroforestería, bosques y cambio climático, restauración de paisajes, derechos, políticas forestales y mucho más, y en varios idiomas. .
CIFOR-ICRAF genera impacto mediante conocimiento científico de vanguardia. Desarrollamos investigaciones innovadoras, fortalecemos las capacidades de nuestros socios y dialogamos activamente con todas las partes interesadas, aportando el conocimiento más reciente sobre los bosques, árboles, paisajes y personas para la toma de decisiones a escala mundial.
CIFOR-ICRAF aborda retos y oportunidades locales y, al mismo tiempo, ofrece soluciones a los problemas globales relacionados con los bosques, los paisajes, las personas y el planeta.
Aportamos evidencia empírica y soluciones prácticas para transformar el uso de la tierra y la producción de alimentos: conservando y restaurando ecosistemas, respondiendo a las crisis globales del clima, la malnutrición, la pérdida de biodiversidad y la desertificación. En resumen, mejorando la vida de las personas.
CIFOR–ICRAF achieves science-driven impact. We conduct innovative research, strengthen partners’ capacity and actively engage in dialogue with all stakeholders, bringing the latest insights on forests, trees, landscapes and people to global decision making.
CIFOR–ICRAF publishes over 750 publications every year on agroforestry, forests and climate change, landscape restoration, rights, forest policy and much more – in multiple languages.
CIFOR–ICRAF addresses local challenges and opportunities while providing solutions to global problems for forests, landscapes, people and the planet.
We deliver actionable evidence and solutions to transform how land is used and how food is produced: conserving and restoring ecosystems, responding to the global climate, malnutrition, biodiversity and desertification crises. In short, improving people’s lives.
